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| From egg to embryo: observing various stages of development in live zebrafish |
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Author:
Alexander Picker
Description:
The zebrafish Danio rerio is a very useful model organism
for the study of how genes instruct the process of embryonic
development from the stage of a single cell –
the fertilised egg – to a complex, multi-cellular
organism, bearing all major morphological features of
a future adult organism and displaying specified and
differentiated tissues.
This activity will guide you through the early development
of live zebrafish embryos.
Development will be observed by bright-light and fluorescence
microscopy.
You will also be introduced to the use of reporter genes
to visualize and follow embryonic development and you
will compare the mode of cell division in zebrafish
embryos to that of embryos from other species.
Objective:
Introduce the advantages and uses of fish as a model system in research
Basics of zebrafish embryogenesis
Concepts of reporter genes and transgenesis
Biological Model:
Live zebrafish (Danio rerio)
Material:
Tutorial
1: Why use fish as a model system? [PDF]
Tutorial
2: The establishment of the body plan [PDF]
Tutorial
3: What is a reporter gene? [PDF]
Procedure and Protocol [PDF]
Results
[PDF]
Glossary [PDF] |
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