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The Beginning of Life: Sea Urchin fertilisation and development
Authors:
Peter Lenart, Alexandra Manaia, Jan Ellenberg (EMBL)

Description:
In this activity the sea urchin is used as a model animal to gain insight on the process of egg fertilization, the trigger that initiates life.

The initial steps of development are strikingly similar within the animal kingdom. Therefore the information gathered through experimentation in a particular model can be applied to a large span of organisms, from jellyfish to humans. Sea urchins are particularly well suited to study early embryogenesis because fertilization is external, gametes are of considerable size and are produced in large quantities, and the embryos are transparent, allowing direct observation of internal and external structures.

Sea urchin gametes will be mixed together to initiate the complex developmental process leading to the formation of a new organism. You will have the chance to observe, explore and record the fascinating events occurring immediately after fertilization – from the block of polyspermy to the first cell divisions of the embryo and its initial development.

Objective:
Explore the basic concepts underlying the initial steps of embryonic development.

Biological Model:
Sea Urchin

Materials:
Protocol: When sperm meets egg [PDF]
Tutorial 1: Fertilization and block of polyspermy [PDF]
Tutorial 2: Sea urchin early development [PDF]
Results [PDF]
Glossary
Sea urchin websites
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This project is funded by the EC, DG Research, with the contract number QLG7-CT-2002-30573 under the programme Quality of Life and Management of Living Resources.
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